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rabbit anti phospho fyn  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti phospho fyn
    Rabbit Anti Phospho Fyn, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 296 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+phospho+fyn/bio_rxiv__2025__10__03__680254-85-34-38?v=Cell+Signaling+Technology+Inc
    Average 95 stars, based on 296 article reviews
    rabbit anti phospho fyn - by Bioz Stars, 2026-07
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    Ligands of mGlu 2/3 receptors influence expression of phosphorylated Fyn and subunits of N-methyl-D-aspartate receptors (NMDAR) in substantia nigra (SN) in sub-acute 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice. Western blot analysis showed the levels of p-GluN2A tyr1325 (A) , p-GluN2B Tyr1472 (C) and p-Fyn <t>Tyr416</t> (E) in SN of each group. Quantification of ratios of p-GluN2A tyr1325/GluN2A (B) , p-GluN2B Tyr1472/GluN2B (D) . * p < 0.05, ** p < 0.01; # p < 0.05 vs. LY354740 + saline group. N = 3 per group in (B) ; N = 4 per group in (D) . Quantification of the ratio of p-Fyn Tyr416/Fyn (F) of each group. * p < 0.05, ** p < 0.01; # p < 0.05 vs. the normal + saline group. N = 3. Relative band intensities were calculated as a ratio of the phosphorylated protein to total protein. The data above were expressed as % of the normal + saline group and error bars represent SEM. Western blot analysis showed the levels of p-GluN2A tyr1325, p-Fyn Tyr416 (G) and p-GluN2B Tyr1472 (I) in SN of each group. (J) Quantification of ratios of p-GluN2A tyr1325/GluN2A and p-GluN2B Tyr1472/GluN2B of each group. * p < 0.05, ** p < 0.01. N = 3. (H) Quantification of ratios of p-Fyn Tyr416/Fyn of each group. * p < 0.05; && p < 0.01 vs. the other two groups. N = 3. Relative band intensities were calculated as a ratio of the phosphorylated protein to total protein. Data in (H, J) were expressed as % of the saline group and error bars represent SEM.
    Anti Phosphorylated Fyn, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Ligands of mGlu 2/3 receptors influence expression of phosphorylated Fyn and subunits of N-methyl-D-aspartate receptors (NMDAR) in substantia nigra (SN) in sub-acute 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice. Western blot analysis showed the levels of p-GluN2A tyr1325 (A) , p-GluN2B Tyr1472 (C) and p-Fyn Tyr416 (E) in SN of each group. Quantification of ratios of p-GluN2A tyr1325/GluN2A (B) , p-GluN2B Tyr1472/GluN2B (D) . * p < 0.05, ** p < 0.01; # p < 0.05 vs. LY354740 + saline group. N = 3 per group in (B) ; N = 4 per group in (D) . Quantification of the ratio of p-Fyn Tyr416/Fyn (F) of each group. * p < 0.05, ** p < 0.01; # p < 0.05 vs. the normal + saline group. N = 3. Relative band intensities were calculated as a ratio of the phosphorylated protein to total protein. The data above were expressed as % of the normal + saline group and error bars represent SEM. Western blot analysis showed the levels of p-GluN2A tyr1325, p-Fyn Tyr416 (G) and p-GluN2B Tyr1472 (I) in SN of each group. (J) Quantification of ratios of p-GluN2A tyr1325/GluN2A and p-GluN2B Tyr1472/GluN2B of each group. * p < 0.05, ** p < 0.01. N = 3. (H) Quantification of ratios of p-Fyn Tyr416/Fyn of each group. * p < 0.05; && p < 0.01 vs. the other two groups. N = 3. Relative band intensities were calculated as a ratio of the phosphorylated protein to total protein. Data in (H, J) were expressed as % of the saline group and error bars represent SEM.

    Journal: Frontiers in Pharmacology

    Article Title: LY354740 Reduces Extracellular Glutamate Concentration, Inhibits Phosphorylation of Fyn/NMDARs, and Expression of PLK2/pS129 α-Synuclein in Mice Treated With Acute or Sub-Acute MPTP

    doi: 10.3389/fphar.2020.00183

    Figure Lengend Snippet: Ligands of mGlu 2/3 receptors influence expression of phosphorylated Fyn and subunits of N-methyl-D-aspartate receptors (NMDAR) in substantia nigra (SN) in sub-acute 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice. Western blot analysis showed the levels of p-GluN2A tyr1325 (A) , p-GluN2B Tyr1472 (C) and p-Fyn Tyr416 (E) in SN of each group. Quantification of ratios of p-GluN2A tyr1325/GluN2A (B) , p-GluN2B Tyr1472/GluN2B (D) . * p < 0.05, ** p < 0.01; # p < 0.05 vs. LY354740 + saline group. N = 3 per group in (B) ; N = 4 per group in (D) . Quantification of the ratio of p-Fyn Tyr416/Fyn (F) of each group. * p < 0.05, ** p < 0.01; # p < 0.05 vs. the normal + saline group. N = 3. Relative band intensities were calculated as a ratio of the phosphorylated protein to total protein. The data above were expressed as % of the normal + saline group and error bars represent SEM. Western blot analysis showed the levels of p-GluN2A tyr1325, p-Fyn Tyr416 (G) and p-GluN2B Tyr1472 (I) in SN of each group. (J) Quantification of ratios of p-GluN2A tyr1325/GluN2A and p-GluN2B Tyr1472/GluN2B of each group. * p < 0.05, ** p < 0.01. N = 3. (H) Quantification of ratios of p-Fyn Tyr416/Fyn of each group. * p < 0.05; && p < 0.01 vs. the other two groups. N = 3. Relative band intensities were calculated as a ratio of the phosphorylated protein to total protein. Data in (H, J) were expressed as % of the saline group and error bars represent SEM.

    Article Snippet: The primary antibodies were: rabbit anti-tyrosine hydroxylase (TH) (Proteintech, 25859-1-AP); mouse anti-polo-like kinase 2 (PLK2) (Santa Cruz; sc-374643); rabbit anti- pS129 α-synuclein (GeneTex; GTX54991); rabbit anti-phospho-Fyn Tyr416 (Cell Signaling Technology; CST2101); mouse anti-Fyn rabbit (Santa Cruz; sc-434); rabbit anti-GluN2A (phospho Y1325) (Abcam; ab16646); rabbit anti-GluN2B (phospho Tyr1472) (Cell Signaling Technology; CST4208); rabbit anti-GluN2A (Genetex; GTX134064); rabbit anti-GluN2B (Genetex; GTX109713); mouse anti-glial fibrillary acidic protein (GFAP) (Servicebio, GB12096); rabbit anti-β-actin (Antgene; ANT 010), rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Antgene; ANT012).

    Techniques: Expressing, Western Blot, Saline

    mGlu 2/3 receptor ligands regulate expression of phosphorylation of Fyn/N-methyl-D-aspartate receptors (NMDAR) in acute 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mice models. Immunoblots and relevant histograms show expression of phosphorylated NMDAR subunit GluN2A at Tyr1325 (A, B), GluN2B at Tyr1472 (B, C), Fyn at Tyr416 (D, F) in substantia nigra (SN) of acute MPTP-treated mice with different doses of LY354740. The band intensity of particular target proteins was normalized to GluN2A, GluN2B or Fyn levels. * p < 0.05, ** p < 0.01. N = 4. Western blots represent the expression of phosphorylated NMDAR subunit GluN2A at Tyr1325 (G, H), GluN2B at Tyr1472 (G, I) and Fyn at Tyr416 (E, J) in acute MPTP-treated mice with saline, LY341495 or LY354740. The band intensity of particular target proteins was normalized to GluN2A, GluN2B or Fyn levels. * p < 0.05, ** p < 0.01 vs. the other two groups. N = 3. Data in (B, D) were expressed as % of the normal + saline group, and data in (E, G) were expressed as % of the MPTP + saline group. Error bars represent SEM.

    Journal: Frontiers in Pharmacology

    Article Title: LY354740 Reduces Extracellular Glutamate Concentration, Inhibits Phosphorylation of Fyn/NMDARs, and Expression of PLK2/pS129 α-Synuclein in Mice Treated With Acute or Sub-Acute MPTP

    doi: 10.3389/fphar.2020.00183

    Figure Lengend Snippet: mGlu 2/3 receptor ligands regulate expression of phosphorylation of Fyn/N-methyl-D-aspartate receptors (NMDAR) in acute 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mice models. Immunoblots and relevant histograms show expression of phosphorylated NMDAR subunit GluN2A at Tyr1325 (A, B), GluN2B at Tyr1472 (B, C), Fyn at Tyr416 (D, F) in substantia nigra (SN) of acute MPTP-treated mice with different doses of LY354740. The band intensity of particular target proteins was normalized to GluN2A, GluN2B or Fyn levels. * p < 0.05, ** p < 0.01. N = 4. Western blots represent the expression of phosphorylated NMDAR subunit GluN2A at Tyr1325 (G, H), GluN2B at Tyr1472 (G, I) and Fyn at Tyr416 (E, J) in acute MPTP-treated mice with saline, LY341495 or LY354740. The band intensity of particular target proteins was normalized to GluN2A, GluN2B or Fyn levels. * p < 0.05, ** p < 0.01 vs. the other two groups. N = 3. Data in (B, D) were expressed as % of the normal + saline group, and data in (E, G) were expressed as % of the MPTP + saline group. Error bars represent SEM.

    Article Snippet: The primary antibodies were: rabbit anti-tyrosine hydroxylase (TH) (Proteintech, 25859-1-AP); mouse anti-polo-like kinase 2 (PLK2) (Santa Cruz; sc-374643); rabbit anti- pS129 α-synuclein (GeneTex; GTX54991); rabbit anti-phospho-Fyn Tyr416 (Cell Signaling Technology; CST2101); mouse anti-Fyn rabbit (Santa Cruz; sc-434); rabbit anti-GluN2A (phospho Y1325) (Abcam; ab16646); rabbit anti-GluN2B (phospho Tyr1472) (Cell Signaling Technology; CST4208); rabbit anti-GluN2A (Genetex; GTX134064); rabbit anti-GluN2B (Genetex; GTX109713); mouse anti-glial fibrillary acidic protein (GFAP) (Servicebio, GB12096); rabbit anti-β-actin (Antgene; ANT 010), rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Antgene; ANT012).

    Techniques: Expressing, Phospho-proteomics, Western Blot, Saline